Serial Dilution Calculatorv1.0.0

A researcher needs to generate a 7-point, 10-fold serial dilution of a cDNA stock for qPCR absolute quantification. The utility calculates volumes for each step, suggests a plate layout, and warns about common errors like incomplete mixing.

An immunologist sets up an ELISA with a capture antibody at 2 μg/mL from a 1 mg/mL stock. The tool computes the serial dilution series for the standard antigen, provides a pipetting scheme, and outputs required volumes for coating and blocking steps.

A pharmacologist prepares a 10-point, 2-fold dilution of a chemotherapeutic agent from 100 μM to 0.2 μM for an IC50 determination. The calculator generates a pipetting table for a 96-well plate and highlights pitfalls like solvent toxicity.

A microbiologist creates a 12-point, 2-fold serial dilution of an antibiotic from 64 μg/mL to 0.03 μg/mL in a 96-well plate for MIC testing. The protocol includes a plate layout suggestion and warnings about edge effects.

A screening scientist programs a liquid handler to perform a 384-well plate serial dilution of a compound library. The tool outputs a pipetting scheme compatible with automation and flags DMSO concentration limits.

Offer basic serial dilution calculations for free; charge a subscription for exportable PDF protocols, branded templates, and integration with lab inventory systems.

Provide a REST API that lab information management systems (LIMS) can call to generate dilution protocols programmatically. License per lab or per query.

Partner with liquid handler manufacturers (e.g., Hamilton, Tecan) to embed the calculator as a built-in module in their run-preparation software.